SFPQ Polyklonaler Antikörper

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• KD/KO Validated

SFPQ Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 15585-1-AP

Synonyme

100 kDa DNA pairing protein, hPOMp100, POMP100, PSF, PTB associated splicing factor, SFPQ

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Galerie der Validierungsdaten

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  WB analysis using 15585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15585-1-AP

  WB result of SFPQ antibody (15585-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFPQ transfected HeLa cells.

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  WB analysis of mouse brain using 15585-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15585-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15585-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15585-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15585-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 15585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 15585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 15585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15585-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15585-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15585-1-AP (SFPQ antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15585-1-AP (SFPQ antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15585-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15585-1-AP (SFPQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BxPC-3-Zellen, humanes Hirngewebe, Jurkat-Zellen, Maushirngewebe, MCF-7-Zellen, PC-3-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 4 publications below

Produktinformation

15585-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA SFPQ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFPQ fusion protein Ag7181
• Vollständiger Name: splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC051192
• Gene symbol: SFPQ
• Gene ID (NCBI): 6421
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ encompasses 707 amino acids and has a molecular weight of 76 kDa, although it typically migrates on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel at an apparent molecular weight of ∼100 kDa. Proteolytic cleavage products of apparent molecular weights of 47 and 68 kDa, and an alternatively spliced form of 669 amino acids, have also been described in various cell types. (PMID: 25832716). Splicing Factor Proline and Glutamine rich (SFPQ) as the most significant intron-retaining transcript across diverse ALS-causing mutations (VCP, SOD1 and FUS). SFPQ protein binds extensively to its retained intron, which exhibits high cytoplasmic abundance in VCP mutation compared with controls. Crucially, the protein is less abundant in the nuclei of VCP mutation cultures and is ultimately lost from nuclei of MNs in mouse models (SOD1mu and VCP mutation transgenic mouse models) and human sporadic ALS post-mortem samples. In summary, our study implicates SFPQ IR and nuclear loss as general molecular hallmarks of familial and sporadic ALS.

Publikationen

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	IF	Hepatology; Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.; Authors - Zhixiang Hu
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human	WB,RIP,IF	Oncogene; AR-induced long non-coding RNA LINC01503 facilitates proliferation and metastasis via the SFPQ-FOSL1 axis in nasopharyngeal carcinoma.; Authors - Shi-Wei He