SFMBT2 Polyklonaler Antikörper

SFMBT2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 25256-1-AP

Synonyme

KIAA1617, SFMBT2

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Galerie der Validierungsdaten

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  WB analysis using 25256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25256-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 25256-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausmilzgewebe, NIH/3T3-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25256-1-AP bindet in WB, ELISA SFMBT2 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFMBT2 fusion protein Ag19379
• Vollständiger Name: Scm-like with four mbt domains 2
• Berechnetes Molekulargewicht: 894 aa, 101 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC152430
• Gene symbol: SFMBT2
• Gene ID (NCBI): 57713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SFMBT2 (Scm like with four mbt domains 2), also named as KIAA1617. It is located in Nucleus and ubiquitous expression in thyroid and ovary. The calculated molecular weight of SFMBT2 is 100 kDa. Although mammalian SFMBTs have similar structural features with PcG protein dSFMBT, biological function and target genes of SFMBT have been not investigated well. Lee K and Na W investigated biological function of human SFMBT2 that interacted with YY1 using AR (androgen receptor)-negative DU145 prostate cancer cells. They found that human SFMBT2 represses HOXB13 gene expression via its association with methylated histones H3 and H4 that are transcriptional repression marks. Furthermore, their findings indicate that SFMBT2 may be involved in regulation of cell growth of DU145 prostate cancer cells (PMID: 23385818).

Publikationen

Species	Application	Title
mouse		Cell Stem Cell; Overcoming Intrinsic H3K27me3 Imprinting Barriers Improves Post-implantation Development after Somatic Cell Nuclear Transfer.; Authors - Le-Yun Wang
		Int J Mol Med; SFMBT2 positively regulates SOX9 and chondrocyte proliferation.; Authors - Safdar Hussain