SETD7 Polyklonaler Antikörper

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• KD/KO Validated

SETD7 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 24840-1-AP

Synonyme

H3 K4 HMTase SETD7, KIAA1717, KMT7, Lysine N methyltransferase 7, SET7, SET7/9, SET9, SETD7

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Galerie der Validierungsdaten

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  WB analysis using 24840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 24840-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24840-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24840-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 24840-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24840-1-AP (SETD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, Maushirngewebe, Mausnierengewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

24840-1-AP bindet in WB, IHC, IF, CoIP, ELISA SETD7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SETD7 fusion protein Ag18219
• Vollständiger Name: SET domain containing (lysine methyltransferase) 7
• Berechnetes Molekulargewicht: 366 aa, 41 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC121055
• Gene symbol: SETD7
• Gene ID (NCBI): 80854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

Publikationen

Species	Application	Title
human	IHC,WB	EBioMedicine; Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients.; Authors - Baojun Duan
human	WB	Oncotarget; SETD7 is a prognosis predicting factor of breast cancer and regulates redox homeostasis.; Authors - Run Huang ; KD Validated
mouse	CoIP	FASEB J; SETD7 mediates the vascular invasion in articular cartilage and chondrocytes apoptosis in osteoarthriis.; Authors - Jia Xiaoshi
human	WB	Exp Cell Res; Proteomic analysis of DZIP3 interactome and its role in proliferation and metastasis in gastric cancer cells; Authors - Rongfang Qiu
human	WB	Med Oncol; DDB1 regulates the activation-induced apoptosis of T cells via downregulating the expression of histone methyltransferase SETD7; Authors - Ruirong Wu
human	CoIP	Histol Histopathol; ZKSCAN5 activates LAPTM5 expression by recruiting SETD7 to promote metastasis in pancreatic ductal adenocarcinoma; Authors - Yong Yang