SESN1 Polyklonaler Antikörper

SESN1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 21668-1-AP

Synonyme

p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1

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Galerie der Validierungsdaten

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  WB analysis using 21668-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21668-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21668-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21668-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, K-562-Zellen, Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 2 publications below

Produktinformation

21668-1-AP bindet in WB, IHC, ELISA SESN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SESN1 fusion protein Ag16462
• Vollständiger Name: sestrin 1
• Berechnetes Molekulargewicht: 551 aa, 64 kDa
• Beobachtetes Molekulargewicht: 66-68 kDa
• GenBank-Zugangsnummer: BC112036
• Gene symbol: SESN1
• Gene ID (NCBI): 27244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).

Publikationen

Species	Application	Title
mouse	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Science; Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.; Authors - Andrew L Cangelosi
		Diabetes; PMID: 25377878; Authors - Rongya Tao
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner; Authors - Sujuan Liu
human	WB,IHC	Osteoarthritis Cartilage; Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.; Authors - T Shen
human	WB	BMC Cancer; Inhibition of mTORC1 through ATF4-induced REDD1 and Sestrin2 expression by Metformin.; Authors - Se-Kyeong Jang