SERPING1/C1 Inactivator Monoklonaler Antikörper

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• KD/KO Validated

SERPING1/C1 Inactivator Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1B11B11

Kat-Nr. : 66882-1-Ig

Synonyme

C1 esterase inhibitor, C1 Inh, C1 inhibiting factor, C1IN, C1INH, C1NH, HAE1, HAE2, Plasma protease C1 inhibitor, Serpin G1, SERPING1, SERPING1/C1 Inactivator

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Galerie der Validierungsdaten

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  WB analysis using 66882-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66882-1-Ig (SERPING1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, human plasma, pig plasma, rat serum
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

66882-1-Ig bindet in WB, IF, ELISA SERPING1/C1 Inactivator und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SERPING1/C1 Inactivator fusion protein Ag16782
• Vollständiger Name: serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
• Berechnetes Molekulargewicht: 500 aa, 55 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC011171
• Gene symbol: SERPING1
• Gene ID (NCBI): 710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein.

Publikationen

Species	Application	Title
human	WB	iScience; Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH; Authors - Simin Yao ; KD Validated