SERPINE2 Monoklonaler Antikörper

SERPINE2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1E11F12

Kat-Nr. : 66203-1-Ig

Synonyme

GDN, Glia derived nexin, Peptidase inhibitor 7, PI 7, PI7, PN 1, PN1, PN-1, PNI, Protease nexin 1, Protease nexin I, Serpin E2, SERPINE2

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Galerie der Validierungsdaten

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  WB analysis using 66203-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 66203-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66203-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 66203-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 66203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 66203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 66203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 66203-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SERPINE2 antibody (66203-1-Ig, Clone: 1E11F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: hTERT-RPE1-Zellen, C6-Zellen, HEK-293-Zellen, Neuro-2a-Zellen, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

66203-1-Ig bindet in WB, IHC, IF, ELISA SERPINE2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SERPINE2 fusion protein Ag1830
• Vollständiger Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC015663
• Gene symbol: SERPINE2
• Gene ID (NCBI): 5270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma.; Authors - Xiyang Zhang
human	IHC	Oncol Lett; Expression pattern of human SERPINE2 in a variety of human tumors.; Authors - Ying Yang
mouse	WB,IHC	Theranostics; Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma; Authors - Lu-Ning Qin