Serpin C1/Antithrombin III Monoklonaler Antikörper

Serpin C1/Antithrombin III Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2B2E5

Rezensionen (2)

Kat-Nr. : 66052-1-Ig

Synonyme

Antithrombin, Antithrombin III, AT3, ATIII, Serpin C1, SERPINC1

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Galerie der Validierungsdaten

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  WB analysis of L02 using 66052-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66052-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66052-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66052-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 66052-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin C1/Antithrombin III antibody (66052-1-Ig, Clone: 2B2E5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of L02 using 66052-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed L02 cells using 66052-1-Ig(Antithrombin III antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, humanes Hirngewebe, humanes Hodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

66052-1-Ig bindet in WB, IHC, IF, ELISA Serpin C1/Antithrombin III und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Serpin C1/Antithrombin III fusion protein Ag9880
• Vollständiger Name: serpin peptidase inhibitor, clade C (antithrombin), member 1
• Berechnetes Molekulargewicht: 464 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC022309
• Gene symbol: SERPINC1
• Gene ID (NCBI): 462
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

Publikationen

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
mouse	WB	Front Oncol; Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene; Authors - Jun Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

Antithrombin III Monoclonal antibody giving a very clear sharp bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer cells

FH

Susmita (Verified Customer) (09-17-2020)

The antibody works good in mentioned dilution for WB and IHC

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 WB; 1:500 IHC

Serpin C1/Antithrombin III Antibody Western Blot,Immunohistochemistry validation (1:1000 WB; 1:500 IHC dilution) in (Cat no:66052-1-Ig)