SERPINA10 Monoklonaler Antikörper

SERPINA10 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

5B3C9

Kat-Nr. : 60113-1-Ig

Synonyme

PZI, Serpin A10, SERPINA10, ZPI

---

Galerie der Validierungsdaten

• 

  WB analysis using 60113-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60113-1-Ig (SERPINA10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 60113-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 60113-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 60113-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 60113-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60113-1-Ig (SERPINA10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

• 

  IF Staining of human liver using 60113-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver using 60113-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using SERPINA10 antibody (60113-1-Ig, Clone: 5B3C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Hausschwein-Lebergewebe, L02-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, humanes Lebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1500-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

60113-1-Ig bindet in WB, IHC, IF, ELISA SERPINA10 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SERPINA10 fusion protein Ag2420
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
• Berechnetes Molekulargewicht: 444 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC022261
• Gene symbol: SERPINA10
• Gene ID (NCBI): 51156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINA10 belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis.

Publikationen

Species	Application	Title
human	IHC	Front Oncol; High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.; Authors - Wenwen Guo