SERP1 Polyklonaler Antikörper

SERP1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17807-1-AP

Synonyme

RAMP4, SERP1

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 17807-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17807-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 17807-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17807-1-AP

  IP result of anti-SERP1 (IP:17807-1-AP, 4ug; Detection:17807-1-AP 1:500) with mouse brain tissue lysate 3160 ug.

• 

  IHC staining of human endometrial cancer using 17807-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 17807-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17807-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17807-1-AP (SERP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Maus-Pankreasgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

17807-1-AP bindet in WB, IP, IHC, IF, ELISA SERP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERP1 fusion protein Ag12087
• Vollständiger Name: stress-associated endoplasmic reticulum protein 1
• Berechnetes Molekulargewicht: 66 aa, 7 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC108314
• Gene symbol: SERP1
• Gene ID (NCBI): 27230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stress-associated endoplasmic reticulum (ER) protein 1 (SERP1), also known as ribosome- associated membrane protein 4 (RAMP4), is a Sec61-associated polypeptide that is induced by ER stress [PMID:16705175]. SERP1 interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. It controls glycosylation of major histocompatibility complex class II-associated invariant chains by a translocational pausing mechanism, and its overexpression stabilizes newly synthesized membrane proteins under ER stress by associating with the Sec61 complex [PMID:10601334]. It is suggested SERP1 is involved in the biosynthesis/processing of secretory proteins

Publikationen

Species	Application	Title
		Viruses; A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.; Authors - Jia-Ni Tian
human	IHC	Am J Transl Res; ER stress-related mRNA-lncRNA co-expression gene signature predicts the prognosis and immune implications of esophageal cancer; Authors - Feng Li
human	WB	Cell Rep; Coronavirus subverts ER-phagy by hijacking FAM134B and ATL3 into p62 condensates to facilitate viral replication; Authors - Xuan Tan