SERINC1 Polyklonaler Antikörper

SERINC1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20265-1-AP

Synonyme

KIAA1253, SERINC1, serine incorporator 1, TDE1L, TDE2, TMS 2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20265-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20265-1-AP (SERINC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20265-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20265-1-AP (SERINC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20265-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20265-1-AP (SERINC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 20265-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20265-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20265-1-AP bindet in WB, IHC, IF,ELISA SERINC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: serine incorporator 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: NM_020755
• Gene symbol: SERINC1
• Gene ID (NCBI): 57515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERINC1, also named as KIAA1253, TDE1L and TDE2, belongs to the TDE1 family. SERINC1 enhances the incorporation of serine into phosphatidylserine and sphingolipids. The antibody is specific to SERINC1.