SERCA2,ATP2A2 Monoklonaler Antikörper

SERCA2,ATP2A2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

1F11A11

Rezensionen (2)

Kat-Nr. : 67248-1-Ig

Synonyme

ATP2A2, ATP2B, Calcium pump 2, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, SERCA2, SERCA2,ATP2A2, SERCA2b, SR Ca(2+) ATPase 2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 67248-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67248-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67248-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67248-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67248-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 67248-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 67248-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67248-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67248-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:20000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

67248-1-Ig bindet in WB, IHC, IF, ELISA SERCA2,ATP2A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SERCA2,ATP2A2 fusion protein Ag5052
• Vollständiger Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Berechnetes Molekulargewicht: 1042 aa, 115 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC035588
• Gene symbol: ATP2A2
• Gene ID (NCBI): 488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

Publikationen

Species	Application	Title
mouse	WB	J Cell Physiol; microRNA-151-3p regulates slow muscle gene expression by targeting ATP2a2 in skeletal muscle cells.; Authors - Huan Wei
mouse	WB	Cell Calcium; Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.; Authors - Yu Chen
rat	WB	Ecotoxicol Environ Saf; Fat mass and obesity-associated gene (FTO) hypermethylation induced by decabromodiphenyl ethane causing cardiac dysfunction via glucolipid metabolism disorder.; Authors - Leqiang Gao
human	WB	J Biol Chem; Cryo-EM structure of mouse TRPML2 in lipid nanodiscs; Authors - Xiaojing Song
human	WB	Clin Transl Med; Metabolic rescue ameliorates mitochondrial encephalo-cardiomyopathy in murine and human iPSC models of Leigh syndrome.; Authors - Jin‐Young Yoon
mouse	WB	Int J Biol Macromol; Lysine crotonylation of SERCA2a correlates to cardiac dysfunction and arrhythmia in Sirt1 cardiac-specific knockout mice; Authors - Huan-Xin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susmita (Verified Customer) (06-13-2022)

This antibody is very good for WB analysis.

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: PC cell line

FH

Iram (Verified Customer) (03-11-2022)

Very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000 for western
• Cell Tissue Type: Colon cancer cells