Septin 11 Polyklonaler Antikörper

Septin 11 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14672-1-AP

Synonyme

SEPT11, septin 11, Septin11

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Galerie der Validierungsdaten

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  WB analysis of human brain using 14672-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14672-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14672-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14672-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 14672-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14672-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14672-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14672-1-AP

  IP Result of anti-Septin 11 (IP:14672-1-AP, 4ug; Detection:14672-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human ovary using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14672-1-AP

  Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 14672-1-AP

  Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 14672-1-AP

  Immunofluorescent analysis of HepG2 cells, using Septin 11 antibody 14672-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HeLa using 14672-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Septin 11 (14672-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, HT-1080.Zellen, Maus-Dünndarmgewebe, Maushirngewebe, Maus-Kolongewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Produktinformation

14672-1-AP bindet in WB, RIP, IP, IHC, IF, FC, ELISA Septin 11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Septin 11 fusion protein Ag6342
• Vollständiger Name: septin 11
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC063615
• Gene symbol: SEPT11
• Gene ID (NCBI): 55752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
mouse	IF	Cytoskeleton (Hoboken); Characterization of septin expression in normal and fibrotic kidneys.; Authors - Katharina Neubauer
human	IF,WB	Cell Rep; An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis; Authors - Thomas C Panagiotou