SEPN1 Polyklonaler Antikörper

SEPN1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 55333-1-AP

Synonyme

MDRS1, RSMD1, RSS, SELENON, Selenoprotein N, selenoprotein N, 1, SELN, SEPN1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 55333-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55333-1-AP (SEPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse uterus using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Mauslungengewebe, Maus-Uterusgewebe, Ratten-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below

Produktinformation

55333-1-AP bindet in WB, IHC, ELISA SEPN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: selenoprotein N, 1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_020451
• Gene symbol: SEPN1
• Gene ID (NCBI): 57190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne); Changes and related factors of blood CCN1 levels in diabetic patients; Authors - Zhao-Yu Xiang
human	WB	Antioxidants (Basel); Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco.; Authors - Dian-Guang Zhang
mouse	WB	Sci Rep; Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins.; Authors - Jiayong Tang
mouse	WB	Mar Drugs; Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.; Authors - Chih-Hung Guo
mouse	WB	Front Nutr; Selenoprotein P Regulates Synaptic Zinc and Reduces Tau Phosphorylation.; Authors - Arlene C P Kiyohara
	WB	Br J Nutr; Dietary selenium (Se) sources differentially regulate Se concentration, mRNA and protein expression of representative selenoproteins in various tissues of yellow catfish Pelteobagrus fulvidraco.; Authors - Xiao-Jian Xu