SEMA4C Polyklonaler Antikörper

SEMA4C Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28402-1-AP

Synonyme

FLJ20369, KIAA1739, M SEMA F, SEMA4C, SEMACL1, SEMAF, SEMAI, Semaphorin 4C

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  WB analysis using 28402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 28402-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28402-1-AP

  Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 28402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28402-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28402-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

28402-1-AP bindet in WB, IHC, ELISA SEMA4C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEMA4C fusion protein Ag29143
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
• Berechnetes Molekulargewicht: 833 aa, 93 kDa
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: BC109104
• Gene symbol: SEMA4C
• Gene ID (NCBI): 54910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEMA4C is a member of transmembrane semaphorin proteins which regulate axonal guidance in the developing nervous system. Furthermore, studies have demonstrated that sema4C was also implicated in biological events outside the nervous system, such as cell migration, tumor development, and terminal myogenic differentiation (PMID: 36010604). SEMA4C is highly expressed in breast, lung, cervical, and liver cancers. SEMA4C is involved in oncogenic signaling and is a potential therapeutic target for invasive breast cancer, hepatocellular carcinoma, glioma, and osteosarcoma (PMID: 33177246).

Publikationen

Species	Application	Title
human	WB	Mutat Res; miR-129-2-3p binds SEMA4C to regulate HCC development and inhibit the EMT; Authors - Siyuan Ma ; KD Validated
Human		Histol Histopathol; Altered expression of the Plexin-B2 system in tuberous sclerosis complex and focal cortical dysplasia IIb lesions; Authors - Lu Dai
human	WB	Front Immunol; Integrative analysis of semaphorins family genes in colorectal cancer: implications for prognosis and immunotherapy; Authors - Jiahao Zhu