SEC24A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SEC24A Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 15958-1-AP

Synonyme

SEC24 related protein A, SEC24A

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15958-1-AP

  WB result of SEC24A antibody (15958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC24A transfected HeLa cells.

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  WB analysis using 15958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15958-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15958-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15958-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat thymus using 15958-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 15958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15958-1-AP (SEC24A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15958-1-AP (SEC24A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15958-1-AP

  Immunofluorescent analysis of Hela cells, using SEC24A antibody 15958-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COS-7-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe, Maus-Skelettmuskelgewebe, Maus-Thymusgewebe, NIH/3T3-Zellen, Ratten-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

15958-1-AP bindet in WB, IP, IHC, IF, ELISA SEC24A und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC24A fusion protein Ag8723
• Vollständiger Name: SEC24 family, member A (S. cerevisiae)
• Berechnetes Molekulargewicht: 613 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 120 kDa
• GenBank-Zugangsnummer: BC019341
• Gene symbol: SEC24A
• Gene ID (NCBI): 10802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). Two isoforms of 120 kDa and 66 kDa produced by alternative splicing have been described.

Publikationen

Species	Application	Title
mouse	WB	Nature; The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.; Authors - Jinbo Han
human	IF	Cell Metab; The hypercholesterolemia-risk gene SORT1 facilitates PCSK9 secretion.; Authors - Camilla Gustafsen
mouse	WB	J Clin Invest; The COPII cargo adapter SEC24C is essential for neuronal homeostasis.; Authors - Bo Wang
mouse	WB,IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human,mouse	WB,IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB,IF,IP	J Immunother Cancer; THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.; Authors - Chushu Li ; KD Validated