SEC23IP Polyklonaler Antikörper

SEC23IP Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 20892-1-AP

Synonyme

MSTP053, P125, SEC23 interacting protein, SEC23IP

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Galerie der Validierungsdaten

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  WB analysis using 20892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20892-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20892-1-AP

  IP Result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug.

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  IHC staining of human heart using 20892-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20892-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 20892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 20892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20892-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Mausnierengewebe, U-251-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20892-1-AP bindet in WB, IP, IHC, IF, ELISA SEC23IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC23IP fusion protein Ag14916
• Vollständiger Name: SEC23 interacting protein
• Berechnetes Molekulargewicht: 1000 aa, 111 kDa
• Beobachtetes Molekulargewicht: 125 kDa
• GenBank-Zugangsnummer: BC063800
• Gene symbol: SEC23IP
• Gene ID (NCBI): 11196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).

Publikationen

Species	Application	Title
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu