SDSL Polyklonaler Antikörper

SDSL Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16242-1-AP

Synonyme

L serine deaminase, L threonine dehydratase, SDH 2, SDS RS1, SDSL, Serine dehydratase 2, serine dehydratase like, TDH

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16242-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16242-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 16242-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16242-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16242-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16242-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16242-1-AP (SDSL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16242-1-AP (SDSL antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16242-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16242-1-AP (SDSL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Kolongewebe, humanes Lebergewebe, Mauslebergewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16242-1-AP bindet in WB, IHC, IF,ELISA SDSL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SDSL fusion protein Ag9114
• Vollständiger Name: serine dehydratase-like
• Berechnetes Molekulargewicht: 329 aa, 35 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC009849
• Gene symbol: SDSL
• Gene ID (NCBI): 113675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.