SDHA Monoklonaler Antikörper

SDHA Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

5E10G12

Rezensionen (1)

Kat-Nr. : 66588-1-Ig

Synonyme

FP, SDH2, SDHA, SDHF

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Galerie der Validierungsdaten

• 

  WB analysis using 66588-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66588-1-Ig (SDHA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66588-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human liver cancer using 66588-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 40x lens).

• 

  IF Staining of HepG2 using 66588-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66588-1-Ig (SDHA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:80000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

66588-1-Ig bindet in WB, IHC, IF, ELISA SDHA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SDHA fusion protein Ag7023
• Vollständiger Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 62-73 kDa
• GenBank-Zugangsnummer: BC001380
• Gene symbol: SDHA
• Gene ID (NCBI): 6389
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma; Authors - Lin-Lin Zhou
		Sci China Life Sci; Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins.; Authors - Jian Xiao
human,mouse	WB	Cell Chem Biol; Aberrant human ClpP activation disturbs mitochondrial proteome homeostasis to suppress pancreatic ductal adenocarcinoma.; Authors - Pengyu Wang
human	WB	Bioorg Chem; SAR-guided development of indole-matrine hybrids as potential anticancer agents via mitochondrial stress/cytochrome c/caspase 3 signaling pathway; Authors - Lingyu Li
mouse	WB	bioRxiv; FAM210A Regulates Mitochondrial Translation and Maintains Cardiac Mitochondrial Homeostasis; Authors - Jiangbin Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (07-12-2023)

Great antibody for WB. Very strong signal and clean background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver

SDHA Antibody Western Blot validation (1:10000 dilution) in Mouse liver (Cat no:66588-1-Ig)