SDC2 Monoklonaler Antikörper

SDC2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

3A7B2

Kat-Nr. : 67088-1-Ig

Synonyme

Fibroglycan, HSPG, HSPG1, SDC2, SYND2, syndecan 2

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Galerie der Validierungsdaten

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  WB analysis using 67088-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67088-1-Ig (SDC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, A549-Zellen, HepG2-Zellen, HuH-7-Zellen, Jurkat-Zellen, NCI-H1299-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67088-1-Ig bindet in WB, IHC, IF, ELISA SDC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SDC2 fusion protein Ag5739
• Vollständiger Name: syndecan 2
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 19 kDa, 22 and 48 kDa
• GenBank-Zugangsnummer: BC049836
• Gene symbol: SDC2
• Gene ID (NCBI): 6383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)

Publikationen

Species	Application	Title
pig	IF	Sci Bull (Beijing); Single-cell dynamics of liver development in postnatal pigs; Authors - Lin Rao
human	WB	Cell Oncol (Dordr); Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2.; Authors - Ningye Ma
human	WB	J Cell Mol Med; miR-218 affects the ECM composition and cell biomechanical properties of glioblastoma cells.; Authors - Małgorzata Grabowska
human	IHC	Cancer Cell Int; Identification of an immune-related signature indicating the dedifferentiation of thyroid cells.; Authors - Xuemin Wang
human	WB	Cancer Cell Int; GNG5 is a novel oncogene associated with cell migration, proliferation, and poor prognosis in glioma.; Authors - Wang Zhang
human	WB	Heart Vessels; Downregulation of lncRNA SNHG16 inhibits vascular smooth muscle cell proliferation and migration in cerebral atherosclerosis by targeting the miR-30c-5p/SDC2 axis.; Authors - Sheng-Nan Yang