SCUBE3 Polyklonaler Antikörper

SCUBE3 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 16773-1-AP

Synonyme

CEGF3, SCUBE3

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 16773-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16773-1-AP (SCUBE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16773-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16773-1-AP (SCUBE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16773-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16773-1-AP (SCUBE3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16773-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16773-1-AP (SCUBE3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

16773-1-AP bindet in WB, IHC, ELISA SCUBE3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCUBE3 fusion protein Ag10085
• Vollständiger Name: signal peptide, CUB domain, EGF-like 3
• Berechnetes Molekulargewicht: 992 aa, 109 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC052263
• Gene symbol: SCUBE3
• Gene ID (NCBI): 222663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCUBE3 is a secreted glycoprotein that belongs to the SCUBE family. Members of the SCUBE family are secreted plasma membrane-associated proteins characterised by a N-terminal signal peptide sequence, multiple EGF domains, a N-linked glycosylated spacer region and a C-terminal CUB region. SCUBE3 may play a role in bone cell biology (PMID: 15234972). It may also has a role in the regulation of cardiac growth and remodeling responses (PMID: 17442284). Besides, SCUBE3 has been reported to be involved in lung cancer progression (PMID: 21441952; 23420440).

Publikationen

Species	Application	Title
human	IHC	Gene; Comprehensive and in-depth analysis of microRNA and mRNA expression profile in salivary adenoid cystic carcinoma.; Authors - Nannan Han
human,mouse	WB	Mol Cell Endocrinol; β-catenin/TCF4-induced SCUBE3 upregulation promotes ovarian cancer development via HIF-1 signaling pathway; Authors - Jing Shen ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (10-04-2021)

Calculated band is 109 kd. This antibody recognized a major band at 60 kd. There was a very weak band ~130 kd, but was PNGaseF insensitive, suggesting this band is not a glycosylated form of this protein.

• Applications: Western Blot