SCO2 Polyklonaler Antikörper

SCO2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 21223-1-AP

Synonyme

SCO1L, SCO2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 21223-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21223-1-AP (SCO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21223-1-AP (SCO2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21223-1-AP (SCO2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21223-1-AP (SCO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21223-1-AP (SCO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21223-1-AP (SCO2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21223-1-AP (SCO2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse skeletal muscle using 21223-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using SCO2 antibody (21223-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Herzgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 2 publications below

Produktinformation

21223-1-AP bindet in WB, IHC, IF, ELISA SCO2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCO2 fusion protein Ag15663
• Vollständiger Name: SCO cytochrome oxidase deficient homolog 2 (yeast)
• Berechnetes Molekulargewicht: 266 aa, 30 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC102025
• Gene symbol: SCO2
• Gene ID (NCBI): 9997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human SCO2 is a mitochondrial membrane-bound protein involved in copper supply for the assembly of cytochrome c oxidase in eukaryotes.It is involved in the maintenance of cell copper homeostasis or, limited to HSco2, as a downstream mediator of the Warburg effect, as its expression is regulated by p53(PMID:17850752).This protein belongs to the SCO1/2 family and defects in SCO2 are the cause of fatal infantile cardioencephalomyopathy with cytochrome c oxidase deficiency (FIC).

Publikationen

Species	Application	Title
human	WB	Cell Metab; Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies.; Authors - David Pacheu-Grau
mouse	WB	Cell Death Differ; Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production.; Authors - Kunlun Yin
human	WB	EMBO Mol Med; APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS.; Authors - Alba Signes
human	WB,IHC	Oncotarget; The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.; Authors - Fabao Liu
human	WB,IHC	Int J Mol Sci; SLMP53-1 Inhibits Tumor Cell Growth through Regulation of Glucose Metabolism and Angiogenesis in a P53-Dependent Manner.; Authors - Helena Ramos
mouse	WB	PLoS One; Mitochondrial dysfunction in Pten haplo-insufficient mice with social deficits and repetitive behavior: interplay between Pten and p53.; Authors - Napoli Eleonora E