RPS4X Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPS4X Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, PLA, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14799-1-AP

Synonyme

CCG2, DXS306, ribosomal protein S4, X linked, RPS4, RPS4X, SCAR, SCR10

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14799-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14799-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14799-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14799-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14799-1-AP

  IP Result of anti-RPS4X (IP:14799-1-AP, 3ug; Detection:14799-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 14799-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RPS4X antibody 14799-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe, Maus-Pankreasgewebe, MCF-7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

14799-1-AP bindet in WB, PLA, IP, IHC, IF, ELISA RPS4X und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Frosch, Krallenfrosch (Xenopus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS4X fusion protein Ag6513
• Vollständiger Name: ribosomal protein S4, X-linked
• Berechnetes Molekulargewicht: 263 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC000472
• Gene symbol: RPS4X
• Gene ID (NCBI): 6191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). [PMID:8358435]

Publikationen

Species	Application	Title
mouse	WB	Cell; Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.; Authors - Takashi Mino
mouse	WB	Nat Commun; Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome.; Authors - Sang S Seo
Xenopus	WB, PLA	Elife; Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.; Authors - Max Koppers
human	IHC	Aging (Albany NY); Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue; Authors - Ranran Zhou
human	WB,IHC	BMC Cancer; Low expression of the X-linked ribosomal protein S4 in human serous epithelial ovarian cancer is associated with a poor prognosis.; Authors - Tsofack Serges P SP ; KD Validated
		Biomark Med; Low level of the X-linked ribosomal protein S4 in human urothelial carcinomas is associated with a poor prognosis.; Authors - Éric R Paquet