SCAMP1 Polyklonaler Antikörper

SCAMP1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15327-1-AP

Synonyme

SCAMP, SCAMP1, SCAMP37

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 15327-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15327-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15327-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15327-1-AP

  IP result of anti-SCAMP1 (IP:15327-1-AP, 4ug; Detection:15327-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

• 

  IHC staining of mouse brain using 15327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15327-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15327-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Maus-Pankreasgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

15327-1-AP bindet in WB, IP, IHC, ELISA SCAMP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCAMP1 fusion protein Ag3258
• Vollständiger Name: secretory carrier membrane protein 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC034048
• Gene symbol: SCAMP1
• Gene ID (NCBI): 9522
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP	Int J Mol Sci; Multiomics Analysis of Endocytosis upon HBV Infection and Identification of SCAMP1 as a Novel Host Restriction Factor against HBV Replication.; Authors - Tanzeel Yousaf
human	IHC	Cell Death Dis; MTSS1 and SCAMP1 cooperate to prevent invasion in breast cancer.; Authors - Jayakumar Vadakekolathu
human	WB	Aging (Albany NY); Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.; Authors - Feiyu Mao