SC65 Polyklonaler Antikörper

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• KD/KO Validated

SC65 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15288-1-AP

Synonyme

NOL55, Nucleolar autoantigen No55, P3H4, SC65

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15288-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15288-1-AP (SC65 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15288-1-AP

  WB result of SC65 antibody (15288-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SC65 transfected HeLa cells.

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  WB analysis using 15288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15288-1-AP (SC65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse bladder using 15288-1-AP

  IP Result of anti-SC65 (IP:15288-1-AP, 4ug; Detection:15288-1-AP 1:500) with mouse bladder tissue lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: Maus-Blasengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

15288-1-AP bindet in WB, IP, IHC, IF, ELISA SC65 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SC65 fusion protein Ag7480
• Vollständiger Name: synaptonemal complex protein SC65
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC001047
• Gene symbol: SC65
• Gene ID (NCBI): 10609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SC65, also named as Leprecan-like protein 4 or Nucleolar autoantigen No55, is a 437 amino acid protein, which belongs to the leprecan family. SC65 ia a protein of the interphase nucleolus and tumour-associated autoantigen in patients with prostate cancer.

Publikationen

Species	Application	Title
mouse	WB,IF	PLoS Genet; Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.; Authors - Melissa E Heard ; KO Validated
human, mouse	WB,IF	J Bone Miner Res; Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.; Authors - Gruenwald Katrin K ; KD Validated
human	WB	Cancers (Basel); P3H4 Promotes Malignant Progression of Lung Adenocarcinoma via Interaction with EGFR.; Authors - Chen Fang ; KD Validated
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
human	WB,IHC	Aging (Albany NY); Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer.; Authors - Lin Hao ; KD Validated
human	WB	Sci Rep; Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure; Authors - Takahiro Isono