SATB1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SATB1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15400-1-AP

Synonyme

DNA binding protein SATB1, SATB homeobox 1, SATB1

---

Galerie der Validierungsdaten

• 

  WB analysis of human brain using 15400-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15400-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15400-1-AP

  IP Result of anti-SATB1 (IP:15400-1-AP, 4ug; Detection:15400-1-AP 1:500) with HEK-293 cells lysate 2000ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen, Maushirngewebe, Mausmilzgewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Produktinformation

15400-1-AP bindet in WB, IP, ELISA SATB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SATB1 fusion protein Ag7615
• Vollständiger Name: SATB homeobox 1
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 86-100 kDa
• GenBank-Zugangsnummer: BC001744
• Gene symbol: SATB1
• Gene ID (NCBI): 6304
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epigenetic modifications and dynamic changes in chromatin organization by organizer proteins have recently been shown to play an instrumental role in regulating cancer-promoting genes. Special AT-rich binding protein (SATB1) is a unique type of global regulator that integrates higher-order chromatin organization -withregulation of gene expression. [PMID:23076250,22998183,23121661] SATB1 is a T cell-enriched transcription factor and a chromatin organizer essential for controlling genes that participate in T-cell development and activation. It regulates gene expression by periodically anchoring matrix attachment regions to the nuclear matrix and directly recruiting chromatin-modifying factors. Depending on its posttranslational modifications, SATB1 activates or represses multiple genes.Its expression is regulated by interleukin-4 (IL4) during T helper-2(Th2) cell differentiation[PMID: 20522714]. The calculated molecular weight of SATB1 is 86 kDa, but modified SATB1 is about 100 kDa (PMID: 22879953).

Publikationen

Species	Application	Title
human	WB,IP	Cell Rep; SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites; Authors - Bao Wang ; KO Validated
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
mouse	WB	Mol Neurobiol; SATB1/SLC7A11/HO-1 Axis Ameliorates Ferroptosis in Neuron Cells After Ischemic Stroke by Danhong Injection; Authors - Sikai Zhan
human	WB	Eur Rev Med Pharmacol Sci; Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.; Authors - X-L Zhang
human	WB	Int J Med Sci; SATB1 Knockdown Inhibits Proliferation and Invasion and Decreases Chemoradiation Resistance in Nasopharyngeal Carcinoma Cells by Reversing EMT and Suppressing MMP-9.; Authors - Dongni Zhou ; KD Validated
human	WB	Int J Clin Exp Pathol; Silencing SATB1 influences cell invasion, migration, proliferation, and drug resistance in nasopharyngeal carcinoma.; Authors - Chun-Sheng Ye ; KD Validated