SARS-CoV-2 S protein (319-541 aa) Polyklonaler Antikörper
SARS-CoV-2 S protein (319-541 aa) Polyklonal Antikörper für WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Virus
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 28991-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Recombinant protein |
Erfolgreiche Detektion in ELISA | Recombinant protein |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
28991-1-AP bindet in WB, ELISA SARS-CoV-2 S protein (319-541 aa) und zeigt Reaktivität mit Virus
Getestete Reaktivität | Virus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | SARS-CoV-2 S protein (319-541 aa) fusion protein Ag30689 |
Vollständiger Name | SARS-CoV-2 Spike Protein |
Berechnetes Molekulargewicht | 141 kDa |
GenBank-Zugangsnummer | NC_045512 |
Gene symbol | COVID-19 S Protein |
Gene ID (NCBI) | 43740568 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28991-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 319-541aa.
Protokolle
Produktspezifische Protokolle | |
---|---|
WB protocol for SARS-CoV-2 S protein (319-541 aa) antibody 28991-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |