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SARS-COV-2 NSP5 Polyklonaler Antikörper

SARS-COV-2 NSP5 Polyklonal Antikörper für IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus

Anwendung

IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29286-1-AP

Synonyme

SARS-COV-2 NSP5

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293T cells using SARS-COV-2 NSP5 Polyclonal antibody (29286-1-AP)

    IF Staining of HEK-293T using 29286-1-AP

    Immunofluorescent analysis of 4% PFA fixed HEK293T cells overexpressing ACE2 and TMPRSS using NSP5 antibody (29286-1-AP, green) at dilution of 1:200. Cells were infected with virus (dsRNA, in red, MOI 0.01, 48hpi). The images were credited by Dr. Irene Chen, Gladstone Institute.

  • ELISA experiment using SARS-COV-2 NSP5 Polyclonal antibody (29286-1-AP)

    ELISA experiment using 29286-1-AP

    SARS-CoV-2 Envelope protein Antibody (29286-1-AP) tested by ELISA. SARS-COV-2 NSP5 protein was coated onto microtiter plates at 1 µg/well and then incubated with a dilution series of SARS-COV-2 NSP5 protein Antibody (29286-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHEK-293T-Zellen
Erfolgreiche Detektion in ELISAN/A

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:10-1:100
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

29286-1-AP bindet in IF, ELISA SARS-COV-2 NSP5 und zeigt Reaktivität mit Virus

Getestete Reaktivität Virus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SARS-COV-2 NSP5 fusion protein Ag30934
Vollständiger Name ORF1ab
GenBank-ZugangsnummerYP_009742612
Gene symbol ORF1ab
Gene ID (NCBI) 43740578
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.