SAR1B Polyklonaler Antikörper

SAR1B Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 22292-1-AP

Synonyme

ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB

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Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 22292-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 22292-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22292-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22292-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22292-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22292-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IHC staining of human liver using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22292-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maus-Dünndarmgewebe, Maushirngewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Dünndarmgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

22292-1-AP bindet in WB, IP, IHC, IF, ELISA SAR1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SAR1 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 25-27 kDa
• GenBank-Zugangsnummer: NM_016103
• Gene symbol: SAR1B
• Gene ID (NCBI): 51128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex. The antibody is specific to SAR1B.

Publikationen

Species	Application	Title
human	WB,IP	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen ; KO Validated
mouse	WB,IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
mouse,human	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
rat	WB	J Biol Chem; The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.; Authors - Lee G D Fryer
mouse	WB	J Biol Chem; Small intestine but not liver lysophosphatidylcholine acyltransferase 3 (Lpcat3) deficiency has a dominant effect on plasma lipid metabolism.; Authors - Inamul Kabir