SAR1A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SAR1A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 22291-1-AP

Synonyme

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1

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Galerie der Validierungsdaten

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  WB analysis using 22291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22291-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22291-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 22291-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 22291-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22291-1-AP (SAR1A Antibody) at dilution of 1:50.

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  IHC staining of human skeletal muscle using 22291-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22291-1-AP (SAR1A Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 22291-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SAR1A antibody (22291-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, HT-1080.Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

22291-1-AP bindet in WB, IHC, IF, CoIP, ELISA SAR1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SAR1 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 23-25 kDa
• GenBank-Zugangsnummer: NM_020150
• Gene symbol: SAR1A
• Gene ID (NCBI): 56681
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). The antibody is specific to SAR1A.

Publikationen

Species	Application	Title
human	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen ; KO Validated
human	CoIP	J Cell Sci; A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.; Authors - Jiaxin Sun ; KD Validated
rat	WB	Ann Transl Med; Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.; Authors - Kun Wang
human	WB	Epigenomics; Methylation of TMEM176A, a key ERK signaling regulator, is a novel synthetic lethality marker of ATM inhibitors in human lung cancer.; Authors - Hongxia Li