SAP30 Polyklonaler Antikörper

SAP30 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 27679-1-AP

Synonyme

SAP30

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Galerie der Validierungsdaten

• 

  WB analysis of A549 using 27679-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 27679-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 27679-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HL-60-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

27679-1-AP bindet in WB, IF, ELISA SAP30 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAP30 fusion protein Ag26516
• Vollständiger Name: Sin3A-associated protein, 30kDa
• Berechnetes Molekulargewicht: 220 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23-28 kDa
• GenBank-Zugangsnummer: BC016757
• Gene symbol: SAP30
• Gene ID (NCBI): 8819
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAP30, also named as Sin3 corepressor complex subunit SAP30, is a 220 amino acid protein, which is expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes.

Publikationen

Species	Application	Title
	IF	Development; Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures.; Authors - Sandra Ruiz García
human	WB	Cell Rep; SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns; Authors - Kazuhiro Fukumura