Sam50 Polyklonaler Antikörper

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• KD/KO Validated

Sam50 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 20824-1-AP

Synonyme

CGI 51, OMP85, Sam50, SAMM50, TOB55, TRG 3, TRG3, YNL026W

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Galerie der Validierungsdaten

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  WB analysis using 20824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20824-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 20824-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20824-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 20824-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20824-1-AP (Sam50 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 20824-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20824-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 20824-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 20824-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20824-1-AP (Sam50 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20824-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20824-1-AP (Sam50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, A431-Zellen, Maus-Eierstockgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

20824-1-AP bindet in WB, IHC, IF, ELISA Sam50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sam50 fusion protein Ag14799
• Vollständiger Name: sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 469 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007830
• Gene symbol: SAMM50
• Gene ID (NCBI): 25813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

Publikationen

Species	Application	Title
human	WB	Elife; QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.; Authors - Virginia Guarani
human	WB	Cell Rep; A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.; Authors - Timothy D Martin ; KD Validated
mouse	WB	Aging Cell; 3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex; Authors - Zer Vue
rat	WB	Free Radic Biol Med; Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy.; Authors - Run-Qing Xue
human	WB	J Cell Biol; The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy; Authors - Mehmet Oguz Gok
pig	IF	Elife; Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system; Authors - Dalen Zuidema