SAMD9L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SAMD9L Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 25173-1-AP

Synonyme

C7orf6, DRIF2, KIAA2005, SAMD9L, UEF, UEF1

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Galerie der Validierungsdaten

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  WB analysis using 25173-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 25173-1-AP (SAMD9L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25173-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25173-1-AP (SAMD9L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse spleen using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 25173-1-AP (SAMD9L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of 3T3-L1 using 25173-1-AP

  Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using SAMD9L antibody (25173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of COLO 320 using 25173-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 25173-1-AP (SAMD9L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe, COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hirngewebe, humanes Kolonkarzinomgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: COLO 320-Zellen, 3T3-L1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

25173-1-AP bindet in WB, IHC, IF, ELISA SAMD9L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAMD9L fusion protein Ag18541
• Vollständiger Name: sterile alpha motif domain containing 9-like
• Berechnetes Molekulargewicht: 1584 aa, 185 kDa
• Beobachtetes Molekulargewicht: 160-180 kDa
• GenBank-Zugangsnummer: BC127118
• Gene symbol: SAMD9L
• Gene ID (NCBI): 219285
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sterile alpha motif domain-containing protein 9-like(SAMD9L), also named as C7orf6, DRIF2, KIAA2005 and UEF, is a cytoplasmic protein that acts as a tumor suppressor but also plays a key role in cell proliferation and the innate immune response to viral infection. SAMD9L protein is an endosome fusion facilitator which causes myeloid malignancies in mice similar to human diseases with monosomy 7. The SAMD9L protein also appears to play an important role in the brain, particularly the part of the brain that coordinates movement (the cerebellum). SAMD9L has two isoforms with MW 185 kDa and 47 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Med; Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes.; Authors - Sushree S Sahoo
human	WB	PLoS Pathog; A paralogous pair of mammalian host restriction factors form a critical host barrier against poxvirus infection.; Authors - Xiangzhi Meng ; KO Validated
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB,IF	Brain Commun; New spinocerebellar ataxia subtype caused by SAMD9L mutation triggering mitochondrial dysregulation (SCA49).; Authors - Marc Corral-Juan
human	WB	Exp Cell Res; Viral host range factors antagonize pathogenic SAMD9 and SAMD9L variants; Authors - Stine Gahr
human	IHC	J Inflamm Res; Integrated Analysis Identifies Upregulated SAMD9L as a Potential Biomarker Correlating with the Severity of Primary Sjögren's Syndrome; Authors - Donghai Zhou