SAMD4A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SAMD4A Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17387-1-AP

Synonyme

hSmaug1, KIAA1053, Protein Smaug homolog 1, SAMD4, SAMD4A, Smaug, Smaug 1, Smaug1, SMG, SMGA

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 17387-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17387-1-AP (SAMD4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17387-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17387-1-AP (SAMD4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 17387-1-AP

  IP Result of anti-SAMD4A (IP:17387-1-AP, 4ug; Detection:17387-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human spleen using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17387-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17387-1-AP (SAMD4A antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Mausherzgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

17387-1-AP bindet in WB, IP, IHC, ELISA SAMD4A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAMD4A fusion protein Ag11273
• Vollständiger Name: sterile alpha motif domain containing 4A
• Berechnetes Molekulargewicht: 529 aa, 59 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC021726
• Gene symbol: SAMD4A
• Gene ID (NCBI): 23034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Mol Immunol; SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.; Authors - Yuze Wang ; KO Validated
mouse	WB	J Neuroinflammation; Smad7 in the hippocampus contributes to memory impairment in aged mice after anesthesia and surgery; Authors - Changliang Liu
human	IHC,WB	Tissue Cell; SAMD4A serves as a negative prognostic marker for gastric cancer patients; Authors - Deqin Li ; KD Validated