S100P Monoklonaler Antikörper

S100P Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1G6C3

Kat-Nr. : 67485-1-Ig

Synonyme

MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P

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Galerie der Validierungsdaten

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  WB analysis of BXPC-3 using 67485-1-Ig

  BXPC-3 cells were subjected to SDS PAGE followed by western blot with 67485-1-Ig (S100P antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 67485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 67485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Plazenta-Gewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67485-1-Ig bindet in WB, IHC,ELISA S100P und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S100P fusion protein Ag19115
• Vollständiger Name: S100 calcium binding protein P
• Berechnetes Molekulargewicht: 95 aa, 11 kDa
• Beobachtetes Molekulargewicht: 10 KDa
• GenBank-Zugangsnummer: BC006819
• Gene symbol: S100P
• Gene ID (NCBI): 6286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.