S100 Beta Monoklonaler Antikörper

S100 Beta Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

CloneNo.

1G10F4

Rezensionen (1)

Kat-Nr. : 66616-1-Ig

Synonyme

NEF, Protein S100 B, S 100 protein beta chain, S 100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 66616-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66616-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66616-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human malignant melanoma using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat brain using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66616-1-Ig (S100 beta antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66616-1-Ig (S100 beta antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66616-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 66616-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using S100 Beta antibody (66616-1-Ig, Clone: 1G10F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, C6-Zellen, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Appendizitis-Gewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 10 publications below

Produktinformation

66616-1-Ig bindet in WB, IHC, IF, ELISA S100 Beta und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S100 Beta fusion protein Ag7440
• Vollständiger Name: S100 calcium binding protein B
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC001766
• Gene symbol: S100B
• Gene ID (NCBI): 6285
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.

Publikationen

Species	Application	Title
rat	WB	Acta Neuropathol Commun; Repetitive mild TBI causes pTau aggregation in nigra without altering preexisting fibril induced Parkinson’s-like pathology burden; Authors - Vedad Delic
human,mouse	WB,IF	Cell Mol Life Sci; Adtrp regulates thermogenic activity of adipose tissue via mediating the secretion of S100b.; Authors - Peng Li
mouse	WB	Exp Neurol; Interaction of DCF1 with ATP1B1 induces impairment in astrocyte structural plasticity via the P38 signaling pathway.; Authors - Jiao Wang
rat	IF	Front Pharmacol; Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway.; Authors - Binbin Hu
rat	IHC	ACS Omega; Platelet-Rich Plasma Gel-Loaded Collagen/Chitosan Composite Film Accelerated Rat Sciatic Nerve Injury Repair; Authors - Bo Yuan
rat	IF	Front Neurosci; Nogo-C Inhibits Peripheral Nerve Regeneration by Regulating Schwann Cell Apoptosis and Dedifferentiation.; Authors - Bo Jia

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Clarisse (Verified Customer) (08-08-2022)

This antibody works very well as an astrocyte marker. In the image: in green mouse anti-S100B (1:500 - 66616-1-Ig) and in magenta rabbit anti-S100B (1:500 - 15146-1-AP). The rabbit antibody has a better performance.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human cerebral organoids

S100 Beta Antibody Immunofluorescence validation (1:500 dilution) in Human cerebral organoids (Cat no:66616-1-Ig)