S100A9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A9 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

37

Kat-Nr. : 26992-1-AP

Synonyme

60B8AG, CAGB, Calgranulin B, Calprotectin L1H subunit, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP 14, MRP14, NIF, P14, Protein S100 A9, S100A9

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Galerie der Validierungsdaten

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  WB analysis using 26992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26992-1-AP (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26992-1-AP

  WB result of S100A9 antibody (26992-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A9 transfected MCF-7 cells.

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  IP experiment of MCF-7 using 26992-1-AP

  IP result of anti-S100A9 (IP:26992-1-AP, 4ug; Detection:26992-1-AP 1:1000) with MCF-7 cells lysate 2800 ug.

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  IHC staining of mouse spleen using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 26992-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26992-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100A9 antibody (26992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 26992-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (26992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 26992-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A9 antibody (26992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, MCF-7-Zellen, THP-1-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Tonsillitisgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, A549-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• FC: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

26992-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA S100A9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A9 fusion protein Ag25764
• Vollständiger Name: S100 calcium binding protein A9
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC047681
• Gene symbol: S100A9
• Gene ID (NCBI): 6280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Publikationen

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
mouse	FC	Sci Adv; Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt.; Authors - Wei Cai
human	CoIP	Theranostics; Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.; Authors - Hong Xiang ; KD Validated
mouse	WB,IHC	Theranostics; Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells.; Authors - Genyu Liu
rat	WB	Acta Physiol (Oxf); Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.; Authors - Li Zhang
mouse	WB	Mol Cell Proteomics; Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury; Authors - Yi-Han Lin