S100A8 Monoklonaler Antikörper

S100A8 Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1G11C8

Kat-Nr. : 66853-1-Ig

Synonyme

60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A

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Galerie der Validierungsdaten

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  IHC staining of human tonsillitis using 66853-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66853-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66853-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66853-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66853-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66853-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66853-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using S100A8 antibody (66853-1-Ig, Clone: 1G11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66853-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using S100A8 antibody (66853-1-Ig, Clone: 1G11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

66853-1-Ig bindet in WB, IHC, IF, ELISA S100A8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S100A8 fusion protein Ag8517
• Vollständiger Name: S100 calcium binding protein A8
• Berechnetes Molekulargewicht: 93 aa, 11 kDa
• GenBank-Zugangsnummer: BC005928
• Gene symbol: S100A8
• Gene ID (NCBI): 6279
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

Publikationen

Species	Application	Title
human	IF	J Cell Biol; Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters; Authors - Femmy C Stempels
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
human	IF	Front Immunol; Glomerular Expression of S100A8 in Lupus Nephritis: An Integrated Bioinformatics Analysis.; Authors - Wei Qijiao
human	IHC	J Transl Med; Decoding the colorectal cancer ecosystem emphasizes the cooperative role of cancer cells, TAMs and CAFsin tumor progression; Authors - Rongfang Shen
human	IF	Transl Oncol; Single-cell RNA sequencing analysis revealed cellular and molecular immune profiles in lung squamous cell carcinoma; Authors - Bo Hao
mouse	WB	J Photochem Photobiol B; Hemin protects UVB-induced skin damage through inhibiting keratinocytes apoptosis and reducing neutrophil infiltration; Authors - Fang Bai