S100A4 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A4 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

CloneNo.

2G11B4

Rezensionen (1)

Kat-Nr. : 66489-1-Ig

Synonyme

18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 66489-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66489-1-Ig

  WB result of S100A4 antibody (66489-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A4 transfected HeLa cells.

• 

  WB analysis of A549 using 66489-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 66489-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66489-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66489-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of K-562 using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded k562 slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66489-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66489-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A4 antibody (66489-1-Ig, Clone: 2G11B4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 66489-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66489-1-Ig (S100A4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, A549-Zellen, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Tonsillitisgewebe, K-562-Zellen, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• FC: See 1 publications below

Produktinformation

66489-1-Ig bindet in WB, IHC, IF, FC, ELISA S100A4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S100A4 fusion protein Ag9019
• Vollständiger Name: S100 calcium binding protein A4
• Berechnetes Molekulargewicht: 101 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC016300
• Gene symbol: S100A4
• Gene ID (NCBI): 6275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Publikationen

Species	Application	Title
human	WB	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
mouse	IHC	Cell Death Differ; Upregulation of Wilms' Tumor 1 in epicardial cells increases cardiac fibrosis in dystrophic mice.; Authors - Zhenglong Guo
mouse	IF,FC	PLoS Pathog; S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation.; Authors - Wei Yang
mouse	WB	Cancers (Basel); Usp22 Overexpression Leads to Aberrant Signal Transduction of Cancer-Related Pathways but Is Not Sufficient to Drive Tumor Formation in Mice; Authors - Xianghong Kuang
human	IF	Physiol Genomics; Altered placental ion channel gene expression in preeclamptic high-altitude pregnancies; Authors - Colleen G Julian
rat	WB,IF	J Cell Mol Med; Polydatin inhibits ZEB1-invoked epithelial-mesenchymal transition in fructose-induced liver fibrosis.; Authors - Xiaojuan Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

I have some reservations about the antibody because of its relatively low molecular weight. We attempted to create separate gels with 10% and 12% concentrations and made an effort to run the gels for a limited time to ensure that the entire protein remained within the original gels and membranes. While we were able to detect several bands, their positions lower in the gel led to less than ideal band shapes.

• Applications: Western Blot
• Primary Antibody Dilution: 1：500
• Cell Tissue Type: Lung cancer and breast cancer cell lines

S100A4 Antibody Western Blot validation (1：500 dilution) in Lung cancer and breast cancer cell lines (Cat no:66489-1-Ig)