S100A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16027-1-AP

Synonyme

Protein S100 A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100, S100 alpha, S100A, S100A1

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Galerie der Validierungsdaten

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  WB analysis of human heart using 16027-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16027-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human malignant melanoma using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human malignant melanoma using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens)..

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  IHC staining of human tonsillitis using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16027-1-AP (S100A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16027-1-AP (S100A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human appendicitis using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16027-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human ovary cancer using 16027-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human ovary cancer using 16027-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Appendizitis-Gewebe, humanes Nierengewebe, humanes Ovarialkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, humanes Tonsillitisgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: human ovary cancer tissue

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

16027-1-AP bindet in WB, IHC, IF, ELISA S100A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A1 fusion protein Ag8871
• Vollständiger Name: S100 calcium binding protein A1
• Berechnetes Molekulargewicht: 94 aa, 11 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC014392
• Gene symbol: S100A1
• Gene ID (NCBI): 6271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	IF	Biomaterials; Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.; Authors - Xianzhen Dong
human	IHC	Mol Neurobiol; Anti-Apoptotic Effect of IGF1 on Schwann Exposed to Hyperglycemia is Mediated by Neuritin, a Novel Neurotrophic Factor.; Authors - Lingfei Yan
rat	WB	Front Pharmacol; Reynoutrin Improves Ischemic Heart Failure in Rats Via Targeting S100A1.; Authors - Wenkai Yang ; KD Validated
human	WB	Circ Heart Fail; S100A1 in Human Heart Failure: Lack of Recovery Following LVAD Support.; Authors - Mosi K Bennett
rat	WB,IF	Mol Med Rep; S100A1 expression is increased in spinal cord injury and promotes inflammation, oxidative stress and apoptosis of PC12 cells induced by LPS via ERK signaling; Authors - Ye Bai
rat	IF	Sci Adv; An injectable and adaptable hydrogen sulfide delivery system for modulating neuroregenerative microenvironment; Authors - Xianzhen Dong