Red fluorescent protein Monoklonaler Antikörper

Red fluorescent protein Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

rekombinanten Protein und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

1E3C5

Kat-Nr. : 67378-1-Ig

Synonyme

GFP-like chromoprotein, Red fluorescent protein eqFP611, RFP, RFP tag

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 67378-1-Ig

  RFP tagged protein were subjected to SDS PAGE followed by western blot with 67378-1-Ig (RFP antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ag27669

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

67378-1-Ig bindet in WB, IF, ELISA Red fluorescent protein und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: Hefe, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Red fluorescent protein fusion protein Ag27669
• Vollständiger Name: Red fluorescent protein
• Berechnetes Molekulargewicht: 26 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins. Red fluorescent protein from Entacmaea quadricolor. Abs(max)=559 nm; Exhibits a smaller absorbance peak at 525 nm. It has a strong fluorescence emission spectrum which peaks at 611 nm. This antibody can be used to detect the RFP tagged (Entacmaea quadricolor) protein.

Publikationen

Species	Application	Title
yeast	WB	bioRxiv; Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins; Authors - Alaina H Willet
mouse	IF	EMBO Rep; Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome; Authors - Fakun Cao
yeast	WB	Mol Biol Cell; Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins; Authors - Alaina H Willet
	WB	Mol Plant Pathol; Reticulons 3 and 6 interact with viral movement proteins; Authors - Jens Tilsner
	WB	Autophagy; The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple; Authors - Runmin Che