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Rabbit IgG control Polyklonaler Antikörper

Rabbit IgG control Polyklonal Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Anwendung

FC

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-30000

Synonyme

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin

Galerie der Validierungsdaten

Filter:
  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite®555-conjugated Rabbit IgG control Polyclo (CL555-30000)

    FC experiment of PC-3 using CL555-30000

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®555 Anti-Phospho-STK11/LKB1 (Thr189) Rabbit Recombinant antibody (CL555-80127), or 0.13 ug CoraLite®555 Rabbit IgG control (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-30000 bindet in FC Rabbit IgG control und zeigt Reaktivität mit

Getestete Reaktivität
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name Rabbit IgG control
Gene symbol
Gene ID (NCBI)
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Reinigungsmethode N/A
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.