RXRA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RXRA Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Kat-Nr. : 21218-1-AP

Synonyme

NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA, RXRα

---

Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 21218-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HCT 116 using 21218-1-AP

  HCT 116 lysates prepared and IP of RXRA performed using 1.0 μg of 21218-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IP experiment of MCF-7 using 21218-1-AP

  IP Result of anti-RXRA (IP:21218-1-AP, 4ug; Detection:21218-1-AP 1:600) with MCF-7 cells lysate 800ug.

• 

  IHC staining of human kidney using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 21218-1-AP

  Immunofluorescent analysis of Hela cells, using RXRA antibody 21218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen, HCT 116-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Magenkrebsgewebe, humanes Schilddrüsenkarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below
• RIP: See 1 publications below

Produktinformation

21218-1-AP bindet in WB, RIP, IP, IHC, IF, ChIP, ELISA RXRA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RXRA fusion protein Ag15651
• Vollständiger Name: retinoid X receptor, alpha
• Berechnetes Molekulargewicht: 462 aa, 51 kDa
• Beobachtetes Molekulargewicht: 50-54 kDa
• GenBank-Zugangsnummer: BC110998
• Gene symbol: RXRA
• Gene ID (NCBI): 6256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver and also expressed in the lungs, kidneys, and heart. It can recognize the mature 54 kDa RXRA and the truncated 44 kDa RXRA (PMID: 20541701).

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Nat Genet; Comprehensive characterization of protein-protein interactions perturbed by disease mutations.; Authors - Feixiong Cheng
human	WB	Sci Adv; Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.; Authors - Leiming Wang
human	ChIP	Lab Anim (NY); Animal models of male subfertility targeted on LanCL1-regulated spermatogenic redox homeostasis.; Authors - Chao Huang
human	WB	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng
human	WB	Cancer Res; Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.; Authors - Ming Cui