RWDD1 Polyklonaler Antikörper

RWDD1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 17036-1-AP

Synonyme

CGI 24, DFRP2, PTD013, RWD domain containing 1, RWDD1

---

Galerie der Validierungsdaten

• 

  WB analysis using 17036-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17036-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17036-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17036-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17036-1-AP (RWDD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse thymus using 17036-1-AP

  IP Result of anti-RWDD1 (IP:17036-1-AP, 4ug; Detection:17036-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

• 

  IHC staining of mouse kidney using 17036-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17036-1-AP (RWDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maus-Thymusgewebe, Ratten-Thymusgewebe
• Erfolgreiche IP: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

17036-1-AP bindet in WB, IP, IHC, ELISA RWDD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RWDD1 fusion protein Ag10525
• Vollständiger Name: RWD domain containing 1
• Berechnetes Molekulargewicht: 243 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28-36 kDa
• GenBank-Zugangsnummer: BC015802
• Gene symbol: RWDD1
• Gene ID (NCBI): 51389
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RWDD1(RWD domain-containing protein 1) is also named as DFRP2(DRG family-regulatory protein 2) and belongs to the RWDD1/GIR2 family. RWDD1 is a thymus aging-related protein which contains an RWD domain at its N-terminus and it is expressed in both thymocytes and thymic epithelial cells and located primarily in the cytoplasm. This protein expression in thymus is decreased in aged and oxidatively stressed mice (PMID:18954556). RWDD1 has a calculated molecular mass of 28 kDa, and apparent molecular mass of 32-36 kDa due to its high acidic amino acid composition that results in the slowed migration behavior in electrophoresis (PMID: 30278355, 18954556).

Publikationen

Species	Application	Title
mouse	WB,IHC	Mol Cell Endocrinol; RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation.; Authors - Gr?tsch Helga H