RUNX2 Rekombinanter Antikörper

RUNX2 Rekombinant Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1G5

Rezensionen (1)

Kat-Nr. : 82636-2-RR

Synonyme

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

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Galerie der Validierungsdaten

• 

  WB analysis using 82636-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82636-2-RR (RUNX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of ROS1728 using 82636-2-RR

  IP result of anti-RUNX2(IP:82636-2-RR, 4ug; Detection:82636-2-RR 1:500) with ROS1728 cells lysate 2080 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, C2C12-Zellen
• Erfolgreiche IP: ROS1728-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

82636-2-RR bindet in WB, IP, ELISA RUNX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: runt-related transcription factor 2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-60 kDa
• GenBank-Zugangsnummer: NM_004348
• Gene symbol: RUNX2
• Gene ID (NCBI): 860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.

Publikationen

Species	Application	Title
human	WB	Life Sci; Circ_0114581 promotes osteogenic differentiation of BMSCs via the MiR-155-5p/HNRNPA3 axis; Authors - Hao Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (11-26-2023)

Worked well for ADSCs at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ADSCs