RUNX1T1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RUNX1T1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15494-1-AP

Synonyme

AML1T1, CBFA2T1, CDR, Cyclin D related protein, Eight twenty one protein, ETO, MTG8, MTG8b, Protein CBFA2T1, Protein ETO, Protein MTG8, RUNX1T1, ZMYND2

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 15494-1-AP

  WB result of RUNX1T1 antibody (15494-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RUNX1T1 transfected HEK-293 cells.

• 

  WB analysis using 15494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15494-1-AP (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15494-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15494-1-AP (RUNX1T1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15494-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15494-1-AP (RUNX1T1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15494-1-AP

  IP Result of anti-RUNX1T1 (IP:15494-1-AP, 4ug; Detection:15494-1-AP 1:800) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of mouse brain using 15494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15494-1-AP (RUNX1T1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 15494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15494-1-AP (RUNX1T1 Antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

15494-1-AP bindet in WB, IP, IHC, IF, ELISA RUNX1T1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RUNX1T1 fusion protein Ag7892
• Vollständiger Name: runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 64-69 kDa
• GenBank-Zugangsnummer: BC005850
• Gene symbol: RUNX1T1
• Gene ID (NCBI): 862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. Various transcript of the fusion gene has been reported. RUNX1T1 exists some isoforms with MV 68, 67,64, 48 and 44 kDa. The calcualted molecular weight of RUNX1T1 is 67 kDa, but modified RUNX1T1is about 70-75 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Res; FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis.; Authors - Xu Zhao
sheep	WB,IF	Int J Mol Sci; Characterization of RUNX1T1, an Adipogenesis Regulator in Ovine Preadipocyte Differentiation.; Authors - Kaiping Deng ; KD Validated
human	WB	J Biol Chem; Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners; Authors - Yidan Xu