RTKN2 Polyklonaler Antikörper

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• KD/KO Validated

RTKN2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17458-1-AP

Synonyme

PLEKHK1, rhotekin 2, RTKN2

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 17458-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lymph using 17458-1-AP

  mouse lymph tissue were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 17458-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17458-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 17458-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HEK-293-Zellen, Maus-Lymphgewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

17458-1-AP bindet in WB, IHC, IF, ELISA RTKN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RTKN2 fusion protein Ag11098
• Vollständiger Name: rhotekin 2
• Berechnetes Molekulargewicht: 163aa,19 kDa; 609aa,69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC025765
• Gene symbol: RTKN2
• Gene ID (NCBI): 219790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Rep; Cellular hormetic response to 27-hydroxycholesterol promotes neuroprotection through AICD induction of MAST4 abundance and kinase activity.; Authors - Brendan Gongol ; KD Validated
human	WB,IHC	J Viral Hepat; Depletion of circ_0088046 suppressed cell growth and motility of hepatocellular carcinoma via circ_0088046-miR-1299-RTKN2 ceRNA pathway; Authors - Li Zhang