RRS1 Polyklonaler Antikörper

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• KD/KO Validated

RRS1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15329-1-AP

Synonyme

KIAA0112, RRR, RRS1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 15329-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15329-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15329-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15329-1-AP (RRS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15329-1-AP

  IP Result of anti-RRS1 (IP:15329-1-AP, 4ug; Detection:15329-1-AP 1:800) with K-562 cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

15329-1-AP bindet in WB, IP, ELISA RRS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRS1 fusion protein Ag7030
• Vollständiger Name: RRS1 ribosome biogenesis regulator homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC001811
• Gene symbol: RRS1
• Gene ID (NCBI): 23212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao ; KD Validated
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	WB	Biol Direct; Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism; Authors - Akihiro Yamashita