RRM2B,p53R2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RRM2B,p53R2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 18005-1-AP

Synonyme

p53R2, RRM2B, RRM2B,p53R2

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Galerie der Validierungsdaten

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  WB analysis using 18005-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18005-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18005-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18005-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 18005-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, MDA-MB-453s-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

18005-1-AP bindet in WB, ELISA RRM2B,p53R2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRM2B,p53R2 fusion protein Ag12499
• Vollständiger Name: ribonucleotide reductase M2 B (TP53 inducible)
• Berechnetes Molekulargewicht: 351 aa, 41 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC108261
• Gene symbol: RRM2B
• Gene ID (NCBI): 50484
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Ther; DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.; Authors - Fangfang Zhang ; KD Validated