RRM1-Specific Polyklonaler Antikörper

RRM1-Specific Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19686-1-AP

Synonyme

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1, RRM1-Specific

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Galerie der Validierungsdaten

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  WB analysis of A549 using 19686-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19686-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19686-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 19686-1-AP

  Immunofluorescent analysis of HepG2 cells, using RRM1 antibody 19686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19686-1-AP bindet in WB, IF, ELISA RRM1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribonucleotide reductase M1
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: NM_001033
• Gene symbol: RRM1
• Gene ID (NCBI): 6240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RRM1, also named as RR1, belongs to the ribonucleoside diphosphate reductase large chain family. RRM1 provides the precursors necessary for DNA synthesis. RRM1 catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.RRM1 is a key enzyme in DNA synthesis: low level expression of RRM1 is associated with poor survival. RRM1 is a prognostic marker of NSCLC. The antibody is specfic to RRM1.