RRAGC Polyklonaler Antikörper

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• KD/KO Validated

RRAGC Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 26989-1-AP

Synonyme

GTPase interacting protein 2, GTR2, Rag C, RAGC, Ras related GTP binding C, RRAGC, TIB929

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 26989-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26989-1-AP

  WB result of RRAGC antibody (26989-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRAGC transfected Jurkat cells.

• 

  WB analysis of HeLa using 26989-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26989-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 26989-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 26989-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

26989-1-AP bindet in WB, ELISA RRAGC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRAGC fusion protein Ag25780
• Vollständiger Name: Ras-related GTP binding C
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC016668
• Gene symbol: RRAGC
• Gene ID (NCBI): 64121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB	Genet Med; De novo missense variants in RRAGC lead to a fatal mTORopathy of early childhood; Authors - Margot R F Reijnders